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Essential preparation steps include: Hand hygiene – wash hands thoroughly with soap and water or use alcohol-based hand sanitiser Gather supplies – B12 ampoule, sterile syringe and needle, alcohol wipes, sharps container, and cotton wool or gauze Check the medication – verify the correct drug, strength, and expiry date

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In addition, Nox4 facilitates cardiac-related adaptation to chronic stress by activating Nrf2, which increases concentrations of GSH and, consequently, increases the GSH/GSSG ratio [158]